The story of a butterfly that explains how species evolve.

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People often wonder or rather doubt how new species are formed. Some people doubt the whole phenomenon of evolution because it's "a theory of evolution". However, in reality, evolution happens and we can find evidence of it in nature. Here, for instance, a species of butterfly in Amazon region was found to have evolved from the hybridization of two other species of butterflies. Interestingly, the descendants of all these three lineages of butterflies coexist in same geographical region and two of these occasionally mate with each other.
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TBE buffer (Tris Borate EDTA)

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Usage:

  1. To prepare agarose gel for separating or analyzing DNA fragments.
  2. As electrolyte during electrophoresis of above DNA fragments.
 

component solubility (g/100ml) (30 ⁰C)
Tris base 50
Boric acid 6.23
EDTA see here





Composition of TBE buffer (10 X, 1litre)



Component Amount 
Tris base 108 g
Boric acid 55 g
0.5 M EDTA 40 ml



Looking at the solubility of boric acid, you can see that preparation of 50X or 100X TBE buffer is not possible.

Working concentration of TBE buffer is 0.5X, i.e. you can prepare agarose gel in 0.5X TBE buffer and also run the gel in 0.5X TBE buffer.

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