Long term storage of Cyanobacteria
I wanted to make stocks of cyanobacteria for my work and I found the following method most comfortable.
- Make a solution of 10% v/v methanol using the bacterial medium as solvent NOT just water and autoclave.
- While maintaining sterile conditions, take 0.5 ml of log-phase cyanobacterial culture in a 1.5ml vial.
- To this add 0.5ml of 10% methanol solution.
- Mix by inversion.
- Store the vial at -20 degree Celsius.